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Trace evidence

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Title: Trace evidence  
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Subject: Forensic science, Forensic materials engineering, Forensic polymer engineering, Forensic photography, Outline of forensic science
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Trace evidence

Trace evidence is created when objects contact. Material is often transferred by heat or induced by contact friction.

The importance of trace evidence in criminal investigations was shown by Dr. Edmond Locard in the early 20th Century. Since then, forensic scientists use trace evidence to reconstruct crimes, and to describe the people, places and things involved in them. Studies of homicides published in the forensic science literature show how trace evidence is used to solve crimes. Trace evidence is important in accident investigation, where movement of one part against another will often leave a tell-tale mark. Such analysis is of great use in forensic engineering.


  • Examples 1
  • Protection 2
  • Analysis 3
  • Problems 4
  • See also 5
  • References 6


Examples of typical trace evidence in criminal cases include glove prints, hairs, cosmetics, Lipsticks,[1] plant fibers, mineral fibers, synthetic fibers, glass, paint chips, soils, footprints, botanical materials, gunshot residue, explosives residue, and volatile hydrocarbons (arson evidence). For such evidence to be useful, it must be compared to similar items from suspects, but particular care is necessary to ensure a thorough analysis.

Skid mark from faulty ladder.

Ladder feet often leave a trace pattern on the ground, so showing how the ladder moved and caused an accident to the user. Skid marks from tires are often critical in determining the sequence of events before and during a car crash. Vehicular accident reconstruction relies on such marks to estimate vehicle speed before and during an accident, as well as braking and impact forces. Fabric prints of clothing worn by pedestrians in the paint and/or road grime of the striking vehicle can match a specific vehicle involved in a hit-and-run collision. Such traces are also known as "witness marks", especially in engineering and may be critical in understanding how a product failed. A typical witness mark could be an impact depression which broke a product, especially if that mark can be matched to the product which made the impact such as a hammer or nail. Such marks are also commonly encountered in criminal cases, and include bite marks, puncture marks, bullet holes etc.


The first preservation is to photograph in situ, and then remove the objects showing key traces, protect them, and analyse under controlled laboratory conditions.

Many techniques are used in the protection of trace evidence from criminal investigations, although all must be photographed as soon as possible, and while still in place. Samples may be collected by shaking, brushing, taping, vacuuming, swabbing and hand picking. Great care may be needed to prevent contamination with other substances (such as natural oil and sweat on the hand of the collector). In some cases, such as with oil or grease, a solvent extraction can be used to collect the evidence for analysis. The method used for collection is generally dependent on both the type of evidence and from where or what sort of object it is being collected.

Trace Evidence is also found in much smaller amounts at crime scenes.


Close-up of broken fuel pipe using optical microscopy
Droplets of human blood. The droplets are round and show no splattering, indicating they impacted relatively slowly, in this case from a height of two feet.

Analysis of trace materials most often begins with a visual examination of the evidence usually involving macrophotography. This is then usually followed by microscopic analysis, of which a number of different types are available depending on the type of material to be analysed, such as a stereomicroscope, scanning electron microscope (SEM) or comparison microscope. SEM is especially useful because X-ray analysis can be conducted on selected areas of the sample, so is a form of microanalysis. It is useful where chemical residues can show unusual elements present which may indicate chemical attack of the product. A car accident caused by a diesel fuel leak, for example, showed traces of sulfur on the cracked tube indicative of attack by sulfuric acid from the battery.

Gunshot residue may be identified by elemental analysis using atomic absorption or with a scanning electron microscope equipped with an energy dispersive spectroscope. Small amounts of explosives, volatile hydrocarbons, and other chemicals are identified with the use of analytical instruments, such as gas chromatography, mass spectrometry, and infrared spectroscopy, all of which separate out the components of the chemicals.

Similar comments apply to damaged items from an accident scene, but care is needed in ensuring that the sample is not damaged by the testing, or sampling for testing. Such nondestructive testing must always be used first before considering destructive methods which involve taking small samples from the item for more detailed tests, such as spectroscopic analysis. Use of all such methods must be done in consultation with other experts and the relevant authorities, such as lawyers on both sides of a case.


False positives and contamination by subsequent handling or nearby objects (e.g. mixing of blood from victim and attacker), for example, are problems owing to the presence of many common substances and the necessity of human involvement in the collection of trace evidence. Both can occur with DNA traces and fingerprints. Partial fingerprints are even more vulnerable to false positives. Samples from accidents or crimes should therefore be protected as much as possible by enclosure in a sealable container as soon as possible, after an incident is under investigation.[2][3]

See also


  1. ^ Joshi B, Verma K, Singh J (2013) A Comparison of Red Pigments in Different Lipsticks Using Thin Layer Chromatography (TLC). J Anal Bioanal Techniques 4: 157. doi:10.4172/2155-9872.1000157
  2. ^ Forensic evidence goes on trial
  3. ^ How far should fingerprints be trusted?
  • Mute Witnesses: Trace evidence analysis, by Houck, Max M (Ed),Academic Press (2001).
  • Forensic Materials Engineering: Case Studies by Peter Rhys Lewis, Colin Gagg, Ken Reynolds, CRC Press (2004).
  • The Analysis of Dust Traces, by Locard, Edmomnd, American Journal of Police Science, Vol. 1 (1930), Part I pp. 276–98, Patrt II pp. 401–18, Part III pp. 496–514.
  • Fiber Evidence and The Wayne Williams Trial, by Deadman, Harold A., FBI Law Enforcement Bulletin, March 1984, pp. 13–20, May 1984, pp. 10–19.
  • Trace Evidence-The Invisible Witness, by Petraco, Nicholas, Journal of Forensic Sciences, Volume 31, Jan. 1986, pp. 321–28.
  • Trajectory Reconstruction I: Trace Evidence in Flight, by Petraco, Nicholas and DeForest, Peter, R., Journal of Forensic Sciences, Volume 35, Nov. 1990.
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