World Library  
Flag as Inappropriate
Email this Article

The RNAi Consortium

Article Id: WHEBN0006517795
Reproduction Date:

Title: The RNAi Consortium  
Author: World Heritage Encyclopedia
Language: English
Subject: Massachusetts Institute of Technology, Harvard University
Collection: Biotechnology Companies, Harvard University, Massachusetts Institute of Technology, Research Support Companies
Publisher: World Heritage Encyclopedia
Publication
Date:
 

The RNAi Consortium

The RNAi Consortium, or TRC, is a Sigma-Aldrich and Open Biosystems.

Contents

  • Hairpin selection 1
  • Hairpin vector 2
  • Release 3
  • External links 4

Hairpin selection

A set of candidate hairpins are selected based on the 1st Refseq transcript from each NCBI gene. They should be 21mers, be at least 25bp from start of the coding sequence and no closer than 150bp from its end. Candidates are scored based on various empirical rules (see the Broad Institute's web site for a complete list [1]) and then BLASTed against 2 transcriptome sets. Hairpins that are unique for a Unigene cluster and a RefSeq NM identifier are preferred. Lastly, the candidates are spaced to have 1 hairpin in the 3' untranslated region and 4 in the coding sequence.

Hairpin vector

Selected hairpins are cloned into the vector pLKO1, which is a multipurpose plasmid that can be propagated in bacteria, transfected into mammalian cell lines or used for generation of lentiviruses. It contains resistance genes against ampicillin and puromycin.

Release

Release 1 of the TRC lentiviral shRNA libraries consist of about 35'000 shRNA constructs against 5300 human (25'000 clones) and 2200 mouse genes (10'000 clones). Release 2 of the human shRNA library contained an additional 9'500 clones. Releases occur roughly every quarter. The completing of both mouse and human libraries is envisioned for the end of 2006 or beginning of 2007.

External links

  • TRC homepage at the Broad Institute
  • TRC hairpin selection rules
  • Sigma-Aldrich TRC intro page
  • OpenBiosystems TRC intro page
This article was sourced from Creative Commons Attribution-ShareAlike License; additional terms may apply. World Heritage Encyclopedia content is assembled from numerous content providers, Open Access Publishing, and in compliance with The Fair Access to Science and Technology Research Act (FASTR), Wikimedia Foundation, Inc., Public Library of Science, The Encyclopedia of Life, Open Book Publishers (OBP), PubMed, U.S. National Library of Medicine, National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health (NIH), U.S. Department of Health & Human Services, and USA.gov, which sources content from all federal, state, local, tribal, and territorial government publication portals (.gov, .mil, .edu). Funding for USA.gov and content contributors is made possible from the U.S. Congress, E-Government Act of 2002.
 
Crowd sourced content that is contributed to World Heritage Encyclopedia is peer reviewed and edited by our editorial staff to ensure quality scholarly research articles.
 
By using this site, you agree to the Terms of Use and Privacy Policy. World Heritage Encyclopedia™ is a registered trademark of the World Public Library Association, a non-profit organization.
 


Copyright © World Library Foundation. All rights reserved. eBooks from Project Gutenberg are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.