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Mannan-binding lectin

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Title: Mannan-binding lectin  
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Subject: Lectins, Complement system, Integrin alphaXbeta2, C3b, Blood proteins
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Mannan-binding lectin

Mannose-binding lectin (protein C) 2, soluble

PDB rendering based on 1hup.
Available structures
PDB Ortholog search: PDBe, RCSB
External IDs ChEMBL: GeneCards:
RNA expression pattern
Species Human Mouse
RefSeq (mRNA)
RefSeq (protein)
Location (UCSC)
PubMed search

Mannose-binding lectin (MBL), also called mannose-binding protein or mannan-binding protein (MBP), is a lectin that is instrumental in innate immunity.[1][2]


MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three presumably identical peptide chains of about 30 kDa each.

Although MBL can form several oligomeric forms, there are indications that dimers and trimers are not biologically active and at least a tetramer form is needed for activation of complement.[3]

Genes and polymorphisms

Human MBL2 gene is located on chromosome 10q11.2-q21.[4] Mice have two homologous genes, but in human the first of them was lost. A low level expression of an MBL1 pseudogene 1 (MBL1P1) was detected in liver. The pseudogene encodes a truncated 51-amino acid protein that is homologous to the MBLA isoform in rodents and some primates.[5]

Structural mutations in exon 1 of the human MBL2 gene, at codon 52 (Arg to Cys, allele D), codon 54 (Gly to Asp, allele B) and codon 57 (Gly to Glu, allele C), also independently reduce the level of functional serum MBL by disrupting the collagenous structure of the protein.[6] Furthermore, several nucleotide substitutions in the promoter region of the MBL2 gene at position −550 (H/L polymorphism), −221 (X/Y polymorphism) and −427, −349, −336, del (−324 to −329), −70 and +4 (P/Q polymorphisms) affect the MBL serum concentration. Both the frequency of structural mutations and the promoter polymorphisms that are in strong linkage disequilibrium vary among ethnic groups resulting in seven major haplotypes: HYPA, LYQA, LYPA, LXPA, LYPB, LYQC and HYPD. Differences in the distribution of these haplotypes are the major cause of interracial variations in MBL serum levels. Both HYPA and LYQA are high-producing haplotypes, LYPA intermediate-producing haplotype and LXPA low-producing haplotype, whereas LYPB, LYQC and HYPD are defective haplotypes, which cause a severe MBL deficiency.[7]

Both MBL2 and MBL1P1 genes has been repeatedly hit throughout evolution of primates. The latter silenced eventually by mutations in the glycine residues of the collagen-like region. It has been selectively turned off during evolution through the same molecular mechanisms causing the MBL2 variant alleles in man, suggesting an evolutionary selection for low-producing MBL genes.[6]

Posttranslational modifications

In rat hepatocytes, MBL is synthesized in the rough endoplasmic reticulum. While in Golgi, it undergoes two distinct posttranslational modifications and is assembled into high molecular weight multimeric complexes. The modifications produce MBL in multiple forms of slightly various molecular masses and pI from 5.7 to 6.2.[8] Proteolytic cleavage also resulted in removal of the 20-aa N-terminal signal peptide,[9]and hydroxylation and glycosylation were also detected.[8] Some cysteine residues can be converted to dehydroalanin. [10]


MBL belongs to the class of lectin pathway of the complement system.

Another important function of MBL is that this molecule binds senescent[11] and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.[12][13]


The complement system can be activated through three pathways: the classical pathway, the alternative pathway, and the lectin pathway. One way that the most-recently discovered lectin pathway is activated is through mannose-binding lectin protein. MBL binds to carbohydrates (to be specific, D-mannose and L-fucose residues) found on the surfaces of many pathogens.

For example, MBL has been shown to bind to:


MBL in the blood is complexed with (bound to) another protein, a serine protease called MASP (MBL-associated serine protease). There are three MASPs: MASP-1, MASP-2 and MASP-3, which have protease domains. There are also sMAP (also called MAp19) and MAp44, which do not have protease domains and are thought to be regulatory molecules of MASPs. MASPs also form complexes with ficolins, which are similar to MBL functionally and structurally with the exception that ficolins recognize their targets through fibrinogen-like domains, unlike MBL.

In order to activate the complement system when MBL binds to its target (for example, mannose on the surface of a bacterium), the MASP protein functions to cleave the blood protein C4 into C4a and C4b. The C4b fragments can then bind to the surface of the bacterium, and initiate the formation of a C3-convertase.

The subsequent complement cascade catalyzed by C3-convertase results in creating a membrane attack complex, which causes lysis of the pathogen as well as altered-self in the context of apoptotic and necrotic cells.

MBL/MASP-1 complex also has thrombin-like activity (thrombin clots fibrin to initiate blood clots). Mice that genetically lack MBL or MASP-1/3 (but not MASP-2/sMAP) have prolonged bleeding time in experimental injury models, although mice are seen to be normal if there is no insult to the body.

Clinical significance

It is produced in the [17] The three structural polymorphisms of exon 1 have been reported to cause susceptibility to various common infections, including meningococcal disease.[18][19] However, evidence has been presented that suggests no harmful effect of these variants with regard to mengingococcal disease.[20]

External links


  1. ^ Fraser IP, Koziel H, Ezekowitz RA (1998). "The serum mannose-binding protein and the macrophage mannose receptor are pattern recognition molecules that link innate and adaptive immunity.". Semin. Immunol. 10 (5): 363–72.  
  2. ^ Worthley DL, Bardy PG, Mullighan CG (2005). "Mannose-binding lectin: biology and clinical implications.". Internal medicine journal 35 (9): 548–55.  
  3. ^ Sheriff S, Chang CY, Ezekowitz RA (November 1994). "Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple alpha-helical coiled-coil". Nat. Struct. Biol. 1 (11): 789–94.  
  4. ^ Sastry K, Herman GA, Day L, Deignan E, Bruns G, Morton CC, Ezekowitz RA (October 1989). "The human mannose-binding protein gene. Exon structure reveals its evolutionary relationship to a human pulmonary surfactant gene and localization to chromosome 10". J. Exp. Med. 170 (4): 1175–89.  
  5. ^ Guo N, Mogues T, Weremowicz S, Morton CC, Sastry KN (March 1998). "The human ortholog of rhesus mannose-binding protein-A gene is an expressed pseudogene that localizes to chromosome 10". Mamm. Genome 9 (3): 246–9.  
  6. ^ a b Seyfarth J, Garred P, Madsen HO (2005). "The ‘involution’ of mannose-binding lectin". Human Molecular Genetics 14 (19): 2859–69.  
  7. ^ Online 'Mendelian Inheritance in Man' (OMIM) mannose-binding protein deficiency -614372
  8. ^ a b Colley KJ, Baenziger JU (1987). "Identification of the posttranslational modifications of the core-specific lectin. The core-specific lectin contains hydroxyproline, hydroxylysine, and glucosylgalactosylhydroxylysine residues". J Biol Chem. 262 (21): 10290–5.  
  9. ^ "Mannose-binding protein C precursor [Homo sapiens]". Retrieved 2012-01-03. 
  10. ^ Jensen PH, Laursen I, Matthiesen F, Højrup P (2007). "Posttranslational modifications in human plasma MBL and human recombinant MBL". Biochim. Biophys. Acta - Proteins & Proteomics. 1774: 335–44.  
  11. ^ Tomaiuolo R, Ruocco A, Salapete C, Carru C, Baggio G, Franceschi C, Zinellu A, Vaupel J, Bellia C, Lo Sasso B, Ciaccio M, Castaldo G, Deiana L (March 2012). "Activity of mannose-binding lectin (MBL) in centenarians". Aging Cell. 11 (3): 394–400.  
  12. ^ Ogden CA, deCathelineau A, Hoffmann PR, Bratton D, Ghebrehiwet B, Fadok VA, Henson PM (September 2001). "C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells". J. Exp. Med. 194 (6): 781–95.  
  13. ^ Stuart LM, Takahashi K, Shi L, Savill J, Ezekowitz RA (March 2005). "Mannose-binding lectin-deficient mice display defective apoptotic cell clearance but no autoimmune phenotype". J. Immunol. 174 (6): 3220–6.  
  14. ^ de Jong MA, Vriend LE, Theelen B, Taylor ME, Fluitsma D, Boekhout T, Geijtenbeek TB (March 2010). "C-type lectin Langerin is a beta-glucan receptor on human Langerhans cells that recognizes opportunistic and pathogenic fungi". Mol. Immunol. 47 (6): 1216–25.  
  15. ^ Ji X, Gewurz H, Spear GT (February 2005). "Mannose binding lectin (MBL) and HIV". Mol. Immunol. 42 (2): 145–52.  
  16. ^ B L Herpers, H Endeman, B A W de Jong, B M de Jongh, J C Grutters, D H Biesma, and H van Velzen-Blad. Acute-phase responsiveness of mannose-binding lectin in community-acquired pneumonia is highly dependent upon MBL2 genotypes. Clin Exp Immunol. 2009 Jun;156(3):488-94. PMID 19438602
  17. ^ Worthley DL, Bardy PG, Gordon DL, Mullighan CG (October 2006). "Mannose-binding lectin and maladies of the bowel and liver". World J. Gastroenterol. 12 (40): 6420–8.  
  18. ^ Hibberd, M. L.; Sumiya, M.; Summerfield, J. A.; Booy, R.; Levin, M. (1999). "Association of variants of the gene for mannose-binding lectin with susceptibility to meningococcal disease". The Lancet 353 (9158): 1049.  
  19. ^ Faber, J.; Schuessler, T.; Finn, A.; Murdoch, C.; Zenz, W.; Habermehl, P.; Meyer, C. U.; Zabel, B. U.; Schmitt, H. J.; Zepp, F.; Knuf, M. (2007). "Age-Dependent Association of Human Mannose-Binding Lectin Mutations with Susceptibility to Invasive Meningococcal Disease in Childhood". The Pediatric Infectious Disease Journal 26 (3): 243–246.  
  20. ^ Bradley, D. T.; Bourke, T. W.; Fairley, D. J.; Borrow, R.; Shields, M. D.; Young, I. S.; Zipfel, P. F.; Hughes, A. E. (2012). "Genetic susceptibility to invasive meningococcal disease: MBL2 structural polymorphisms revisited in a large case-control study and a systematic review". International Journal of Immunogenetics: no.  
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