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Amido black 10B

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Title: Amido black 10B  
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Subject: Sodium compounds, Azo dyes, Van Gieson's stain, Aromatic amines, List of dyes
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Amido black 10B

Amido black 10B
Space-filling model of the amido black 10B molecule
IUPAC name
4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt
Other names
Naphthol blue black
Acid Black 1
Acidal Black 10B
Acidal Navy Blue 3BR
Naphthalene Black 10B (see talk page)
Buffalo Black NBR
C.I. 20470
ChemSpider  Y
Jmol-3D images Image
Molar mass 616.49 g·mol−1
Appearance Dark red to black
~ 30 g/l at 20 °C (68 °F)
R-phrases R20, R21, R33
S-phrases S28A, S37, S45
NFPA 704
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
 N  (: Y/N?)

Amido black 10B is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

External links

  • MSDS at Oxford University
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