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Title: Grb2  
Author: World Heritage Encyclopedia
Language: English
Subject: CD28, SOS1, Lymphocyte cytosolic protein 2, MST1R, PDGFRB
Publisher: World Heritage Encyclopedia


Growth factor receptor-bound protein 2
PDB rendering based on 1gri.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols  ; ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2
External IDs ChEMBL: GeneCards:
RNA expression pattern
Species Human Mouse
RefSeq (mRNA)
RefSeq (protein)
Location (UCSC)
PubMed search

Growth factor receptor-bound protein 2 also known as Grb2 is an adaptor protein involved in signal transduction/cell communication. In humans, the GRB2 protein is encoded by the GRB2 gene.[1][2]

The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.[3]


  • Function and expression 1
  • Domains 2
  • Interactions 3
  • See also 4
  • References 5
  • Further reading 6
  • External links 7

Function and expression

Grb2 is widely expressed and is essential for multiple Gads and Grap. Gads and Grap are expressed specifically in hematopoietic cells and function in the coordination of tyrosine kinase mediated signal transduction.


The SH2 domain of Grb2 binds to phosphorylated tyrosine-containing peptides on receptors or scaffold proteins with a preference for pY-X-N-X, where X is generally a hydrophobic residue such as valine (see [6]).

The N-terminal SH3 domain binds to proline-rich peptides and can bind to the Ras-guanine exchange factor SOS.

The C-terminal SH3 domain binds to peptides conforming to a P-X-I/L/V/-D/N-R-X-X-K-P motif that allows it to specifically bind to proteins such as Gab-1.[4]


Grb2 has been shown to interact with:

See also


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  3. ^ "Entrez Gene: GRB2 growth factor receptor-bound protein 2". 
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Further reading

  • Colledge M, Froehner SC (May 1998). "Interaction between the nicotinic acetylcholine receptor and Grb2. Implications for signaling at the neuromuscular junction". Annals of the New York Academy of Sciences 841: 17–27.  
  • Ramesh N, Antón IM, Martínez-Quiles N, Geha RS (Jan 1999). "Waltzing with WASP". Trends in Cell Biology 9 (1): 15–9.  
  • O'Sullivan E, Kinnon C, Brickell P (1999). "Wiskott-Aldrich syndrome protein, WASP". The International Journal of Biochemistry & Cell Biology 31 (3-4): 383–7.  
  • Schlaepfer DD, Hauck CR, Sieg DJ (1999). "Signaling through focal adhesion kinase". Progress in Biophysics and Molecular Biology 71 (3-4): 435–78.  
  • Vidal M, Liu WQ, Gril B, Assayag F, Poupon MF, Garbay C (2004). "[Design of new anti-tumor agents interrupting deregulated signaling pathways induced by tyrosine kinase proteins. Inhibition of protein-protein interaction involving Grb2]". Journal De La Société De Biologie 198 (2): 133–7.  

External links

  • GRB2 Adaptor Protein at the US National Library of Medicine Medical Subject Headings (MeSH)
  • The Grb2 protein page on The SH2 Website
  • GeneCards entry for Grb2
  • Human Protein Resource Database entry for Grb2
  • Grb2 information on iHOP (Information Hyperlinked over Proteins)
  • GRB2 Info with links in the Cell Migration Gateway
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