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Janus Green B

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Subject: Neutral red, Liver cytology, Azo dyes, Aromatic amines, Staining dyes
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Janus Green B

Janus Green B[1]
Ball-and-stick model of the component ions of Janus Green B
IUPAC name
8-(4-Dimethylaminophenyl)diazenyl-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride
Other names
Diazin Green S
Union Green B
ChemSpider  Y
EC number 220-695-6
Jmol-3D images Image
Molar mass 511.06 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
 N  (: Y/N?)

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.[2]

The indicator Janus Green B changes colour according to the amount of oxygen present.[3] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.


  1. ^ Janus Green B, at Sigma-Aldrich
  2. ^ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv fur mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
  3. ^
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