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Title: Lactoferrin  
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Subject: Neisseria, Tej P. Singh, Transferrin, Colostrum, Tears
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Richardson diagram of recombinant human lactoferrin. Based on ​.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols  ; GIG12; HEL110; HLF2; LF
External IDs GeneCards:
EC number
Species Human Mouse
RefSeq (mRNA)
RefSeq (protein)
Location (UCSC)
PubMed search

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is a globular glycoprotein with a molecular mass of about 80 kDa that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin can be purified from milk or produced recombinantly. Human colostrum ("first milk") has the highest concentration, followed by human milk, then cow milk (150 mg/L).[1]

Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses.[1] In particular, lactoferrin provides antibacterial activity to human infants.[2][3] Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.


  • History 1
  • Structure and properties 2
    • Molecular structure 2.1
    • Polymeric forms 2.2
  • Biological functions 3
    • Antibacterial activity 3.1
    • Antiviral activity 3.2
    • Antifungal activity 3.3
    • Bone activity 3.4
    • Interaction with nucleic acids 3.5
    • Enzymatic activity of lactoferrin 3.6
    • Anticarcinogenic activity 3.7
  • Genes of lactoferrin 4
  • Lactoferrin receptor 5
  • Cystic fibrosis 6
  • Nanotechnology 7
  • In Diagnosis 8
  • See also 9
  • References 10
  • External links 11


Occurrence of iron-containing red protein in bovine milk was reported as early as in 1939;[4] however, the protein could not be properly characterized because it could not be extracted with sufficient purity. Its first detailed studies were reported around 1960. They documented the molecular weight, isoelectric point, optical absorption spectra and presence of two iron atoms per protein molecule.[5][6] The protein was extracted from milk, contained iron and was structurally and chemically similar to serum transferrin. Therefore, it was named lactoferrin in 1961, though the name lactotransferrin was used in some earlier publications, and later studies demonstrated that the protein is not restricted to milk. The antibacterial action of lactoferrin was also documented in 1961, and was associated with its ability to bind iron.[7]

Structure and properties

Molecular structure

Lactoferrin is one of the transferrin proteins that transfer iron to the cells and control the level of free iron in the blood and external secretions. It is present in the milk of humans and other mammals,[6] in the blood plasma and neutrophils and is one of the major proteins of virtually all exocrine secretions of mammals, such as saliva, bile, tears and pancreas.[8] Concentration of lactoferrin in the milk varies from 7 g/L in the colostrum to 1 g/L in mature milk.

X-ray diffraction reveals that lactoferrin is based on one polypeptide chain that contains about 700 amino acids and forms two homologous globular domains named N-and C-lobes. N-lobe corresponds to amino acid residues 1–333 and C-lobe to 345–692, and the ends of those domains are connected by a short α-helix.[9][10] Each lobe consists of two subdomains, N1, N2 and C1, C2, and contains one iron binding site and one glycosylation site. The degree of glycosylation of the protein may be different and therefore the molecular weight of lactoferrin varies between 76 and 80 kDa. The stability of lactoferrin has been associated with the high glycosylation degree.[11]

Lactoferrin belongs to the basic proteins, its isoelectric point is 8.7. It exists in two forms: iron-rich hololactoferrin and iron-free apolactoferrin. Their tertiary structures are different; apolactoferrin is characterized by "open" conformation of the N-lobe and the "closed" conformation of the C-lobe, and both lobes are closed in the hololactoferrin.[12]

Each lactoferrin molecule can reversibly bind two ions of iron, zinc, copper or other metals.[13] The binding sites are localized in each of the two protein globules. There, each ion is bonded with six ligands: four from the polypeptide chain (two tyrosine residues, one histidine residue and one aspartic acid residue) and two from carbonate or bicarbonate ions.

Lactoferrin forms reddish complex with iron; its affinity for iron is 300 times higher than that of transferrin.[14] The affinity increases in weakly acidic medium. This facilitates the transfer of iron from transferrin to lactoferrin during inflammations, when the pH of tissues decreases due to accumulation of lactic and other acids.[15] The saturated iron concentration in lactoferrin in human milk is estimated as 10 to 30% (100% corresponds to all lactoferrin molecules containing 2 iron atoms). It is demonstrated that lactoferrin is involved not only in the transport of iron, zinc and copper, but also in the regulation of their intake.[16] Presence of loose ions of zinc and copper does not affect the iron binding ability of lactoferrin, and might even increase it.

Polymeric forms

Both in blood plasma and in secretory fluids lactoferrin can exist in different polymeric forms ranging from monomers to tetramers. Lactoferrin tends to polymerize both in vitro and in vivo, especially at high concentrations.[15] Several authors found that the dominant form of lactoferrin in physiological conditions is a tetramer, with the monomer:tetramer ratio of 1:4 at the protein concentrations of 10−5 M.[17][18][19]

It is suggested that the oligomer state of lactoferrin is determined by its concentration and that polymerization of lactoferrin is strongly affected by the presence of Ca2+ ions. In particular, monomers were dominant at concentrations below 10−10−10−11 M in the presence of Ca2+, but they converted into tetramers at lactoferrin concentrations above 10−9−10−10 M.[17][20] Titer of lactoferrin in the blood corresponds to this particular "transition concentration" and thus lactoferrin in the blood should be presented both as a monomer and tetramer. Many functional properties of lactoferrin depend on its oligomeric state. In particular, monomeric, but not tetrameric lactoferrin can strongly bind to DNA.

Biological functions

Lactoferrin belongs to the innate immune system. Apart from its main biological function, namely binding and transport of iron ions, lactoferrin also has antibacterial, antiviral, antiparasitic, catalytic, anti-cancer, anti-allergic and radioprotecting functions and properties.[21]

Antibacterial activity

Lactoferrin's primary role is to sequester free iron, and in doing so remove essential substrate required for bacterial growth.[22] Antibacterial action of lactoferrin is also explained by the presence of specific peroxides. This affects the membrane permeability and results in the cell breakdown (lysis).[22]

Although lactoferrin also has other antibacterial mechanisms not related to iron, such as stimulation of phagocytosis,[23] the interaction with the outer bacterial membrane described above is the most dominant and most studied.[24] Lactoferrin not only disrupts the membrane, but even penetrates into the cell. Its binding to the bacteria wall is associated with the specific peptide lactoferricin, which is located at the N-lobe of lactoferrin and is produced by in vitro cleavage of lactoferrin with another protein, trypsin.[25][26] A mechanism of the antimicrobial action of lactoferrin has been reported as lactoferrin targets H(+)-ATPase and interferes with proton translocation in the cell membrane, resulting in a lethal effect in vitro.[27]

Lactoferrin prevents the attachment of H. pylori in the stomach, which in turn, aids in reducing digestive system disorders. Bovine lactoferrin has more activity against H. pylori than human lactoferrin.[28]

Antiviral activity

Lactoferrin acts, mostly in vitro, on a wide range of human and animal viruses based on DNA and RNA genomes,[29] including the herpes simplex virus 1 and 2,[30][31] cytomegalovirus,[32] HIV,[31][33] hepatitis C virus,[34][35] hantaviruses, rotaviruses, poliovirus type 1,[36] human respiratory syncytial virus and murine leukemia viruses.[26]

The most studied mechanism of antiviral activity of lactoferrin is its diversion of virus particles from the target cells. Many viruses tend to bind to the lipoproteins of the cell membranes and then penetrate into the cell.[35] Lactoferrin binds to the same lipoproteins thereby repelling the virus particles. Iron-free apolactoferrin is more efficient in this function than hololactoferrin; and lactoferricin, which is responsible for antimicrobial properties of lactoferrin, shows almost no antiviral activity.[29]

Beside interacting with the cell membrane, lactoferrin also directly binds to viral particles, such as the hepatitis viruses.[35] This mechanism is also confirmed by the antiviral activity of lactoferrin against rotaviruses,[26] which act on different cell types.

Lactoferrin also suppresses virus replication after the virus penetrated into the cell.[26][33] Such an indirect antiviral effect is achieved by affecting natural killer cells, granulocytes and macrophages – cells, which play a crucial role in the early stages of viral infections, such as severe acute respiratory syndrome (SARS).[37]

Antifungal activity

Lactoferrin and lactoferricin inhibit in vitro growth of Trichophyton mentagrophytes, which are responsible for several skin diseases such as ringworm.[38] Lactoferrin also acts against the Candida albicans – a diploid fungus (a form of yeast) that causes opportunistic oral and genital infections in humans.[39][40] Fluconazole has long been used against Candida albicans, which resulted in emergence of strains resistant to this drug. However, a combination of lactoferrin with fluconazole can act against fluconazole-resistant strains of Candida albicans as well as other types of Candida: C. glabrata, C. krusei, C. parapsilosis and C. tropicalis.[39] Antifungal activity is observed for sequential incubation of Candida with lactoferrin and then with fluconazole, but not vice versa. The antifungal activity of lactoferricin exceeds that of lactoferrin. In particular, synthetic peptide 1–11 lactoferricin shows much greater activity against Candida albicans than native lactoferricin.[39]

Administration of lactoferrin through drinking water to mice with weakened immune systems and symptoms of gastrointestinal tract. Candida albicans could also be completely eradicated with a mixture containing lactoferrin, lysozyme and itraconazole in HIV-positive patients who were resistant to other antifungal drugs.[42] Such antifungal action when other drugs deem inefficient is characteristic of lactoferrin and is especially valuable for HIV-infected patients.[43] Contrary to the antiviral and antibacterial actions of lactoferrin, very little is known about the mechanism of its antifungal action. Lactoferrin seems to destroy the cell wall and bind the plasma membrane of C. albicans.[40]

Bone activity

Ribonuclease-enriched lactoferrin has been used to examine how lactoferrin affects bone. Lactoferrin has shown to have positive effects on bone turnover. It has aided in decreasing bone resorption and increasing bone formation. This was indicated by a decrease in the levels of two bone resorption markers (deoxypyridinoline and N-telopeptide) and an increase in the levels two bone formation markers (osteocalcin and alkaline phosphatase).[44] It has reduced osteoclast formation, which signifies a decrease in pro-inflammatory responses and an increase in anti-inflammatory responses [45] which indicates a reduction in bone resorption as well.

Interaction with nucleic acids

One of the important properties of lactoferrin is its ability to bind with nucleic acids. The fraction of protein extracted from milk, contains 3.3% RNA,[17] besides, the protein preferably binds to the double-stranded than to the single-stranded DNA. The ability of lactoferrin to bind DNA is used for the isolation and purification of lactoferrin using affinity chromatography with columns containing immobilized DNA-containing sorbents, such as agarose with the immobilized single-stranded DNA.[46]

Enzymatic activity of lactoferrin

Lactoferrin hydrolyzes RNA and exhibits the properties of pyrimidine-specific secretory ribonucleases. In particular, by destroying the RNA genome, milk RNase inhibits reverse transcription of retroviruses that cause breast cancer in mice.[47] Parsi women in West India have the milk RNase level markedly lower than in other groups, and their breast cancer rate is three times higher than average.[48] Thus, ribonucleases of milk, and lactoferrin in particular, might play an important role in pathogenesis of diseases caused by various retroviruses.

Anticarcinogenic activity

The anticancer activity of bovine lactoferrin (bLF) has been demonstrated in experimental lung, bladder, tongue, colon, and liver carcinogeneses on rats, possibly by suppression of phase I enzymes, such as cytochrome P450 1A2 (CYP1A2).[49] Also, in another experiment done on hamsters, bovine lactoferrin decreased the incidence of oral cancer by 50%.[50] Because bLF by far did not show any toxicity and because it's readily available in milk, bLF offers promise as a potential chemopreventive agent for oral cancer. Currently, bLF is used as an ingredient in yogurt, chewing gums, infant formulas, and cosmetics.[50]

Genes of lactoferrin

At least 60 gene sequences of lactoferrin have been characterized in 11 species of mammals.[51] In most species, stop codon is TAA, and TGA in Mus musculus. Deletions, insertions and mutations of stop codons affect the coding part and its length varies between 2,055 and 2,190 nucleotide pairs. Gene polymorphism between species is much more diverse than the intraspecific polymorphism of lactoferrin. There are differences in amino acid sequences: 8 in Homo sapiens, 6 in Mus musculus, 6 in Capra hircus, 10 in Bos taurus and 20 in Sus scrofa. This variation may indicate functional differences between different types of lactoferrin.[51]

In humans, lactoferrin gene LTF is located on the third chromosome in the locus 3q21-q23. In oxen, the coding sequence consists of 17 exons and has a length of about 34,500 nucleotide pairs. Exons of the lactoferrin gene in oxen have a similar size to the exons of other genes of the transferrin family, whereas the sizes of introns differ within the family. Similarity in the size of exons and their distribution in the domains of the protein molecule indicates that the evolutionary development of lactoferrin gene occurred by duplication.[52] Study of polymorphism of genes that encode lactoferrin helps selecting livestock breeds that are resistant to mastitis.[53]

Lactoferrin receptor

The lactoferrin receptor plays an important role in the internalization of lactoferrin; it also facilitates absorption of iron ions by lactoferrin. It was shown that gene expression increases with age in the duodenum and decreases in the jejunum.[54] The moonlighting glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been demonstrated to function as a receptor for lactoferrin.[55]

Cystic fibrosis

The human lung and saliva contain a wide range of antimicrobial compound including lactoperoxidase system, producing hypothiocyanite and lactoferrin, with hypothiocyanite missing in cystic fibrosis patients.[56] Lactoferrin, a component of innate immunity, prevents bacterial biofilm development.[57][58] The loss of microbicidal activity and increased formation of biofilm due to decreased lactoferrin activity is observed in patients with cystic fibrosis.[59] These findings demonstrate the important role of lactoferrin in human host defense and especially in lung.[60]

Lactoferrin with hypothiocyanite has been granted orphan drug status by the EMEA[61] and the FDA.[62]


Lactotransferrin has been used in the synthesis of fluorescent gold quantum clusters, which has potential applications in nanotechnology.[63]

In Diagnosis

Lactoferrin levels in tear fluid have been shown to decrease in dry eye diseases such as Sjogren's syndrome.[64] A rapid, portable test utilizing microfluidic technology has been developed to enable measurement of lactoferrin levels in human tear fluid at the point-of-care with the aim of improving diagnosis of Sjogren's syndrome and other forms of dry eye disease.[65]

See also


  1. ^ a b Sánchez L, Calvo M, Brock JH (1992). "Biological role of lactoferrin". Arch. Dis. Child. 67 (5): 657–61.  
  2. ^ Levin RE, Kalidas S, Gopinadhan P, Pometto A (2006). Food biotechnology. Boca Raton, FL: CRC/Taylor & Francis. p. 1028.  
  3. ^ Animal Breeding: Technology for the 21st Century (Modern Genetics,). Boca Raton: CRC. 1998. p. 191.  
  4. ^ M. Sorensen and S. P. L. Sorensen, Compf. rend. trav. lab. Carlsberg (1939) 23, 55, cited by Groves (1960)
  5. ^ Groves ML (1960). "The Isolation of a Red Protein from Milk". Journal of the American Chemical Society 82 (13): 3345.  
  6. ^ a b Johansson B, Virtanen AI, Tweit RC, Dodson RM (1960). "Isolation of an iron-containing red protein from human milk" (PDF). Acta Chem. Scand. 14 (2): 510–512.  
  7. ^ Naidu AS (2000). Lactoferrin: natural, multifunctional, antimicrobial. Boca Raton: CRC Press. pp. 1–2.  
  8. ^ Birgens HS (1985). "Lactoferrin in plasma measured by an ELISA technique: evidence that plasma lactoferrin is an indicator of neutrophil turnover and bone marrow activity in acute leukaemia". Scand J Haematol 34 (4): 326–31.  
  9. ^ Baker HM, Anderson BF, Kidd RD, Shewry SC, Baker EN (2000). "Lactoferrin three-dimensional structure: a framework for interpreting function". In Shimazaki, Kei-ichi. Lactoferrin: structure, function, and applications: proceedings of the 4th International Conference on Lactoferrin: Structure, Function, and Applications, held in Sapporo, Japan, 18–22 May 1999. Amsterdam: Elsevier.  
  10. ^ Baker EN, Baker HM (2005). "Molecular structure, binding properties and dynamics of lactoferrin". Cell. Mol. Life Sci. 62 (22): 2531–9.  
  11. ^ Håkansson A, Zhivotovsky B, Orrenius S, Sabharwal H, Svanborg C (1995). "Apoptosis induced by a human milk protein". Proc. Natl. Acad. Sci. U.S.A. 92 (17): 8064–8.  
  12. ^ Jameson GB, Anderson BF, Norris GE, Thomas DH, Baker EN (1998). "Structure of human apolactoferrin at 2.0 Å resolution. Refinement and analysis of ligand-induced conformational change". Acta Crystallogr. D Biol. Crystallogr. 54 (Pt 6 Pt 2): 1319–35.  
  13. ^ Levay PF, Viljoen M (1995). "Lactoferrin: a general review". Haematologica 80 (3): 252–67.  
  14. ^ Mazurier J, Spik G (1980). "Comparative study of the iron-binding properties of human transferrins. I. Complete and sequential iron saturation and desaturation of the lactotransferrin". Biochim. Biophys. Acta 629 (2): 399–408.  
  15. ^ a b Broc JHk, De Sousa M (1989). Iron in immunity, cancer, and inflammation. New York: Wiley.  
  16. ^ Shongwe MS, Smith CA, Ainscough EW, Baker HM, Brodie AM, Baker EN (1992). "Anion binding by human lactoferrin: results from crystallographic and physicochemical studies". Biochemistry 31 (18): 4451–8.  
  17. ^ a b c Bennett RM, Davis J (1982). "Lactoferrin interacts with deoxyribonucleic acid: a preferential reactivity with double-stranded DNA and dissociation of DNA-anti-DNA complexes". J. Lab. Clin. Med. 99 (1): 127–38.  
  18. ^ Bagby GC, Bennett RM (1982). "Feedback regulation of granulopoiesis: polymerization of lactoferrin abrogates its ability to inhibit CSA production". Blood 60 (1): 108–12.  
  19. ^ Mantel C, Miyazawa K, Broxmeyer HE (1994). "Physical characteristics and polymerization during iron saturation of lactoferrin, a myelopoietic regulatory molecule with suppressor activity". Adv. Exp. Med. Biol. Advances in, Experimental Medicine and Biology 357: 121–32.  
  20. ^ Furmanski P, Li ZP, Fortuna MB, Swamy CV, Das MR (1989). "Multiple molecular forms of human lactoferrin. Identification of a class of lactoferrins that possess ribonuclease activity and lack iron-binding capacity". J. Exp. Med. 170 (2): 415–29.  
  21. ^ "Lactoferrin: a review" (PDF). Veterinarni Medicina 53 (9): 457. 2008. 
  22. ^ a b Farnaud S, Evans RW (2003). "Lactoferrin—a multifunctional protein with antimicrobial properties". Mol. Immunol. 40 (7): 395–405.  
  23. ^ Xanthou M (1998). "Immune protection of human milk". Biol. Neonate 74 (2): 121–33.  
  24. ^ Odell EW, Sarra R, Foxworthy M, Chapple DS, Evans RW (1996). "Antibacterial activity of peptides homologous to a loop region in human lactoferrin". FEBS Lett. 382 (1–2): 175–8.  
  25. ^ Kuwata H, Yip TT, Yip CL, Tomita M, Hutchens TW (1998). "Bactericidal domain of lactoferrin: detection, quantitation, and characterization of lactoferricin in serum by SELDI affinity mass spectrometry". Biochem. Biophys. Res. Commun. 245 (3): 764–73.  
  26. ^ a b c d Sojar HT, Hamada N, Genco RJ (1998). "Structures involved in the interaction of Porphyromonas gingivalis fimbriae and human lactoferrin". FEBS Lett. 422 (2): 205–8.  
  27. ^ Andrés MT, Fierro JF (2010). "Antimicrobial mechanism of action of transferrins: Selective inhibition of H+-ATPase". Antimicrob. Agents Chemother. 54 (10): 4335–42.  
  28. ^ Editors of Pharmacist's letter, Prescriber's letter, ed. (2007). Natural medicines comprehensive database (10th ed.). Therapeutic Research Faculty. p. 915.  
  29. ^ a b van der Strate BW, Beljaars L, Molema G, Harmsen MC, Meijer DK (2001). "Antiviral activities of lactoferrin". Antiviral Res. 52 (3): 225–39.  
  30. ^ Fujihara T, Hayashi K (1995). "Lactoferrin inhibits herpes simplex virus type-1 (HSV-1) infection to mouse cornea". Arch. Virol. 140 (8): 1469–72.  
  31. ^ a b Giansanti F, Rossi P, Massucci MT, Botti D, Antonini G, Valenti P, Seganti L (2002). "Antiviral activity of ovotransferrin discloses an evolutionary strategy for the defensive activities of lactoferrin". Biochem. Cell Biol. 80 (1): 125–30.  
  32. ^ Harmsen MC, Swart PJ, de Béthune MP, Pauwels R, De Clercq E, The TH, Meijer DK (1995). "Antiviral effects of plasma and milk proteins: lactoferrin shows potent activity against both human immunodeficiency virus and human cytomegalovirus replication in vitro". J. Infect. Dis. 172 (2): 380–8.  
  33. ^ a b Puddu P, Borghi P, Gessani S, Valenti P, Belardelli F, Seganti L (1998). "Antiviral effect of bovine lactoferrin saturated with metal ions on early steps of human immunodeficiency virus type 1 infection". Int. J. Biochem. Cell Biol. 30 (9): 1055–62.  
  34. ^ Azzam HS, Goertz C, Fritts M, Jonas WB (2007). "Natural products and chronic hepatitis C virus". Liver Int. 27 (1): 17–25.  
  35. ^ a b c Nozaki A, Ikeda M, Naganuma A, Nakamura T, Inudoh M, Tanaka K, Kato N (2003). "Identification of a lactoferrin-derived peptide possessing binding activity to hepatitis C virus E2 envelope protein". J. Biol. Chem. 278 (12): 10162–73.  
  36. ^ Arnold D, Di Biase AM, Marchetti M, Pietrantoni A, Valenti P, Seganti L, Superti F (2002). "Antiadenovirus activity of milk proteins: lactoferrin prevents viral infection". Antiviral Res. 53 (2): 153–8.  
  37. ^ Reghunathan R, Jayapal M, Hsu LY, Chng HH, Tai D, Leung BP, Melendez AJ (2005). "Expression profile of immune response genes in patients with Severe Acute Respiratory Syndrome". BMC Immunol. 6: 2.  
  38. ^ Wakabayashi H, Uchida K, Yamauchi K, Teraguchi S, Hayasawa H, Yamaguchi H (2000). "Lactoferrin given in food facilitates dermatophytosis cure in guinea pig models". J. Antimicrob. Chemother. 46 (4): 595–602.  
  39. ^ a b c Lupetti A, Paulusma-Annema A, Welling MM, Dogterom-Ballering H, Brouwer CP, Senesi S, Van Dissel JT, Nibbering PH (2003). "Synergistic activity of the N-terminal peptide of human lactoferrin and fluconazole against Candida species". Antimicrob. Agents Chemother. 47 (1): 262–7.  
  40. ^ a b Viejo-Díaz M, Andrés MT, Fierro JF (2004). "Modulation of in vitro fungicidal activity of human lactoferrin against Candida albicans by extracellular cation concentration and target cell metabolic activity". Antimicrob. Agents Chemother. 48 (4): 1242–8.  
  41. ^ Takakura N, Wakabayashi H, Ishibashi H, Teraguchi S, Tamura Y, Yamaguchi H, Abe S (2003). "Oral lactoferrin treatment of experimental oral candidiasis in mice". Antimicrob. Agents Chemother. 47 (8): 2619–23.  
  42. ^ Masci JR (October 2000). "Complete response of severe, refractory oral candidiasis to mouthwash containing lactoferrin and lysozyme". AIDS 14 (15): 2403–4.  
  43. ^ Kuipers ME, de Vries HG, Eikelboom MC, Meijer DK, Swart PJ (1999). "Synergistic fungistatic effects of lactoferrin in combination with antifungal drugs against clinical Candida isolates". Antimicrob. Agents Chemother. 43 (11): 2635–41.  
  44. ^ Bharadwaj S, Naidu AG, Betageri GV, Prasadarao NV, Naidu AS (September 2009). "Milk ribonuclease-enriched lactoferrin induces positive effects on bone turnover markers in postmenopausal women". Osteoporos Int 20 (9): 1603–11.  
  45. ^ Bharadwaj S, Naidu TA, Betageri GV, Prasadarao NV, Naidu AS (November 2010). "Inflammatory responses improve with milk ribonuclease-enriched lactoferrin supplementation in postmenopausal women". Inflamm. Res. 59 (11): 971–8.  
  46. ^ Rosenmund A, Kuyas C, Haeberli A (1986). "Oxidative radioiodination damage to human lactoferrin". Biochem. J. 240 (1): 239–45.  
  47. ^ McCormick JJ, Larson LJ, Rich MA (1974). "RNase inhibition of reverse transcriptase activity in human milk". Nature 251 (5477): 737–40.  
  48. ^ Das MR, Padhy LC, Koshy R, Sirsat SM, Rich MA (1976). "Human milk samples from different ethnic groups contain RNase that inhibits, and plasma membrane that stimulates, reverse transcription". Nature 262 (5571): 802–5.  
  49. ^ Tsuda H, Sekine K, Fujita K, Ligo M (2002). "Cancer prevention by bovine lactoferrin and underlying mechanisms--a review of experimental and clinical studies". Biochem. Cell Biol. 80 (1): 131–6.  
  50. ^ a b Chandra Mohan KV, Kumaraguruparan R, Prathiba D, Nagini S (September 2006). "Modulation of xenobiotic-metabolizing enzymes and redox status during chemoprevention of hamster buccal carcinogenesis by bovine lactoferrin". Nutrition 22 (9): 940–6.  
  51. ^ a b Jing-Fen Kang, Xiang-Long Li, Rong-Yan Zhou, Lan-Hui Li, Fu-Jun Feng and Xiu -Li Guo (2008). "Bioinformatics Analysis of Lactoferrin Gene for Several Species". Biochemical Genetics 46 (5–6): 312–322.  
  52. ^ Seyfert HM, Tuckoricz A, Interthal H, Koczan D, Hobom G (1994). "Structure of the bovine lactoferrin-encoding gene and its promoter". Gene 143 (2): 265–9.  
  53. ^ O'Halloran F, Bahar B, Buckley F, O'Sullivan O, Sweeney T, Giblin L. (2009). "Characterisation of single nucleotide polymorphisms identified in the bovine lactoferrin gene sequences across a range of dairy cow breeds". Biochimie 91 (1): 68–75.  
  54. ^ Liao Y, Lopez V, Shafizadeh TB, Halsted CH, Lönnerdal B (2007). "Cloning of a pig homologue of the human lactoferrin receptor: expression and localization during intestinal maturation in piglets". Comp Biochem Physiol a Mol Integr Physiol 148 (3): 584–90.  
  55. ^ The multifunctional glycolytic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a novel macrophage lactoferrin receptor. Pooja Rawat, Santosh Kumar, Navdeep Sheokand, Chaaya Iyengar Raje and Manoj Raje. Biochemistry and Cell Biology; 2012, 90(3): 329–338.
  56. ^ Moskwa P, Lorentzen D, Excoffon KJ, Zabner J, McCray PB, Nauseef WM, Dupuy C, Bánfi B (2007). "A novel host defense system of airways is defective in cystic fibrosis". Am. J. Respir. Crit. Care Med. 175 (2): 174–83.  
  57. ^ Singh PK, Schaefer AL, Parsek MR, Moninger TO, Welsh MJ, Greenberg EP (2000). "Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms". Nature 407 (6805): 762–4.  
  58. ^ Singh PK, Parsek MR, Greenberg EP, Welsh MJ (2002). "A component of innate immunity prevents bacterial biofilm development". Nature 417 (6888): 552–5.  
  59. ^ Rogan MP, Taggart CC, Greene CM, Murphy PG, O'Neill SJ, McElvaney NG (2004). "Loss of microbicidal activity and increased formation of biofilm due to decreased lactoferrin activity in patients with cystic fibrosis". J. Infect. Dis. 190 (7): 1245–53.  
  60. ^ Rogan MP, Geraghty P, Greene CM, O'Neill SJ, Taggart CC, McElvaney NG (2006). "Antimicrobial proteins and polypeptides in pulmonary innate defence". Respir. Res. 7 (1): 29.  
  61. ^ "Public summary of positive opinion for orphan designation of hypothiocyanite/lactoferrin for the treatment of cystic fibrosis" (PDF). Pre-authorisation Evaluation of Medicines for Human Use. European Medicines Agency. 2009-09-07. Retrieved 2010-01-23. 
  62. ^ "Meveol: orphan drug status granted by the FDA for the treatment of cystic fibrosis". United States Food and Drug Administration. 2009-11-05. Retrieved 2010-01-23. 
  63. ^ Xavier PL, Chaudhari K, Verma PK, Pal SK, Pradeep T (2010). "Luminescent quantum clusters of gold in transferrin family protein, lactoferrin exhibiting FRET" (PDF). Nanoscale 12 (12): 2769–76.  
  64. ^ Ohashi, Yoshiki; Reiko Ishida; Takashi Kojima; Eiki Goto; Yukihiro Matsumoto; Katsuhiko Watanabe; Naruhiro Ishida; Katsuhiko Nakata; Tsutomu Takeuchi; Kazuo Tsubota (August 2003). "Abnormal Protein Profiles in Tears with Dry Eye Syndrome". American Journal of Ophthalmology 136 (2): 291–9.  
  65. ^ Karns, Kelly; Herr, Amy E (November 2011). "Human Tear Protein Analysis Enabled by an Alkaline Microfluidic Homogeneous Immunoassay". Analytical Chemistry 83 (21): 8115–22.  

External links

  • Uniprot
  • LTF on the National Center for Biotechnology Information
  • FDA Lactoferrin Considered Safe to Fight E. Coli.
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