World Library  
Flag as Inappropriate
Email this Article

MBAS assay

Article Id: WHEBN0029240507
Reproduction Date:

Title: MBAS assay  
Author: World Heritage Encyclopedia
Language: English
Subject: Water chemistry analysis, Colorimetric analysis, Analytical chemistry
Collection: Analytical Chemistry, Anionic Surfactants, Water Pollution
Publisher: World Heritage Encyclopedia
Publication
Date:
 

MBAS assay

A methylene blue active substances assay, or MBAS assay, is a colorimetric analysis test method that uses methylene blue to detect the presence of anionic surfactants (such as a detergent or foaming agent) in a sample of water. An anionic surfactant detected by the color reaction is called a methylene blue active substance (MBAS).[1]

After first acidifying a water sample (with


  • Nollet, Leo M. L. Handbook of Water Analysis. 2nd ed. New York: Marcel Dekker, 2007.

References

  1. ^ Alison L. George, Graham F. White "Optimization of the methylene blue assay for anionic surfactants added to estuarine and marine water" Environmental Toxicology and Chemistry 1999, Volume 18, pages 2232–2236. doi:10.1002/etc.5620181016
  2. ^ a b Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents
  3. ^ a b ASTM D2330 - 02 Standard Test Method for Methylene Blue Active Substances
  4. ^ Standard Methods: 5540 Surfactants

Notes

See also

  • Method 5540B describes surfactant separation by sublation.
  • Method 5540C discusses anionic surfactants as methylene blue active substances (MBAS).
  • Method 5540D discusses nonionic surfactants as cobalt thiocyanate active substances (CTAS).

Standard Methods for the Examination of Water and Wastewater list the following methods used by certified laboratories testing wastewater in the United States:[4]

MBAS assay is an ASTM International standard technique for detecting anionic surfactants.[3] These include carboxylates, phosphates, sulfates, and sulfonates. An MBAS assay alone does not, however, identify specific surfactants. ASTM withdrew the standard (ASTM D2330-02) in 2011 pending a review and update of the method, which was last approved in 2003.[3]

If an anionic surfactant is present, then the cationic methylene blue and the anionic surfactant forms an ion pair, which is extracted into the organic phase. The color of the chloroform increases with increasing concentration of surfactants. [2]

This article was sourced from Creative Commons Attribution-ShareAlike License; additional terms may apply. World Heritage Encyclopedia content is assembled from numerous content providers, Open Access Publishing, and in compliance with The Fair Access to Science and Technology Research Act (FASTR), Wikimedia Foundation, Inc., Public Library of Science, The Encyclopedia of Life, Open Book Publishers (OBP), PubMed, U.S. National Library of Medicine, National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health (NIH), U.S. Department of Health & Human Services, and USA.gov, which sources content from all federal, state, local, tribal, and territorial government publication portals (.gov, .mil, .edu). Funding for USA.gov and content contributors is made possible from the U.S. Congress, E-Government Act of 2002.
 
Crowd sourced content that is contributed to World Heritage Encyclopedia is peer reviewed and edited by our editorial staff to ensure quality scholarly research articles.
 
By using this site, you agree to the Terms of Use and Privacy Policy. World Heritage Encyclopedia™ is a registered trademark of the World Public Library Association, a non-profit organization.
 


Copyright © World Library Foundation. All rights reserved. eBooks from Project Gutenberg are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.