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Protein A

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Title: Protein A  
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Subject: Protein G, Protein L, Repligen, Immunoglobulin Y, Staphylococcus aureus
Collection: Proteins, Staphylococcaceae
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Protein A

Protein A
Structure of a domain of protein A as a three-helix bundle binding to the heavy variable chain of a VH3 human Fab [1] left. Minimized protein A bound to Fc fragment of Rituximab.[2]
Identifiers
Symbol SpA
SCOP 1DEE
SUPERFAMILY 1DEE

Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs. It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis.

Contents

  • History 1
  • Protein A antibody binding 2
  • Other antibody binding proteins 3
  • Role in pathogenesis 4
  • Research 5
  • References 6

History

As a by-product of his work on type-specific staphylococcus antigens, Verwey reported 1940 that a protein fraction prepared from extracts of these bacteria non-specifically precipitated rabbit antisera raised against different staphylococcus types.[3] 1958, Jensen confirmed Verwey’s finding and showed that rabbit pre-immunization sera as well as normal human sera bound to the active component in the staphylococcus extract; he designated this component Antigen A (because it was found in fraction A of the extract) but thought it was a polysaccharide.[4] The misclassification of the protein was the result of faulty tests [5] but it was not long thereafter (1962) that Löfkvist and Sjöquist corrected the error and confirmed that Antigen A was in fact a surface protein on the bacterial wall of certain strains of S. Aureus.[6] The Bergen group from Norway named the protein "Protein A" after the antigen fraction isolated by Jensen.[7]

Protein A antibody binding

Protein A can bind with strong affinity the Fc portion of immunoglobulin of certain species:

Immunoglobulin Species Protein A Binding Human IgG, IgG2, IgG4 Strong

Human IgG3, IgD None

Human IgM, IgA Weak

Mouse IgG1 Weak

Mouse IgG2A, IgG2BIgG3 Strong

Mouse IgM None

Rat IgG1 Weak

Rat IgG2A, IgG2B None

Rat IgG2C Strong

Goat IgG1 Weak

Goat IgG2 Strong

Bovine IgG1 None

Bovine IgG2 Strong

Guinea Pig IgG Strong

Rabbit IgG Strong

Chicken IgG (IgY) None [8]

Other antibody binding proteins

In addition to Protein A, other immunoglobulin-binding bacterial proteins such as Protein G, Protein A/G and Protein L are all commonly used to purify, immobilize or detect immunoglobulins.

Role in pathogenesis

As a pathogen Staphylococcus aureus utilizes Protein A, along with a host of other proteins and surface factors to aid its survival and, thus, virulence. Protein A helps inhibit phagocytic engulfment and acts as an immunological disguise. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence.[9] Higher levels of Protein A in different strains of S. aureus have been associated with nasal carriage of this bacteria.[10]

Research

Recombinant Staphylococcal Protein A is often produced in E. coli for use in immunology and other biological research. One recombinant form of Protein A is called MabSelect.[11] Protein A is often coupled to other molecules such as a fluorescent dye, enzymes, biotin, colloidal gold or radioactive iodine without affecting the antibody binding site. It is also widely utilized coupled to magnetic, latex and agarose beads.

Protein A is often immobilized onto a solid support and used as reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid, or coupled with one of the above markers to detect the presence of antibodies. Immunoprecipitation studies with protein A conjugated to beads are also commonly used to purify proteins or protein complexes indirectly through antibodies against the protein or protein complex of interest.

References

  1. ^ Graille M, Stura EA, Corper AL, Sutton BJ, Taussig MJ, Charbonnier JB, Silverman GJ. (May 2000). protein A domain complexed with the Fab fragment of a human IgM antibody: structural basis for recognition of B-cell receptors and superantigen activity."Staphylococcus aureus"Crystal structure of a . Proc Natl Acad Sci U S A 97 (10): 5399–404.  
  2. ^ Idusogie EE, Presta LG, Gazzano-Santoro H, Totpal K, Wong PY, Ultsch M, Meng YG, Mulkerrin MG. (April 2000). "Mapping of the C1q binding site on rituxan, a chimeric antibody with a human IgG1 Fc". J Immunol 164 (8): 4178–84.  
  3. ^ Verwey, W F (1940). "A type-specific antigenic protein derived from the staphylococcus" (PDF). J. Exp. Med. 71: 635–644. 
  4. ^ Jensen, K (1958). "A normally occurring staphylococcus antibody in human serum". Acta Pathol. Microbiol. Scand. 44: 421–428. 
  5. ^ Dixon, Frank J. (Aug 11, 1982). ADVANCES IN IMMUNOLOGY. Academic Press. p. 158. 
  6. ^ Löfkvist, Thorvald; Sjöquist, John (November 1962). "CHEMICAL AND SEROLOGICAL ANALYSIS OF ANTIGEN PREPARATIONS FROM STAPHYLOCOCCUS AUREUS". Acta Pathologica Microbiologica Scandinavica 56 (3): 295–304. 
  7. ^ Grov, A; Myklestad, B; Oeding, P (1964). "Immunochemical studies on antigen preparations from staphylococcus aureus. 1. Isolation and chemical characterization of Antigen A". Acta Pathol. Microbiol. Scand. (61): 588–596. 
  8. ^ http://www.AnaSpec.com
  9. ^ Goodyear CS, Silverman GJ (May 2003). "Death by a B cell superantigen: In vivo VH-targeted apoptotic supraclonal B cell deletion by a Staphylococcal Toxin". J. Exp. Med. 197 (9): 1125–39.  
  10. ^ Gowrishankar Muthukrishnan, Gerry A. Quinn, Ryan P. Lamers, Carolyn Diaz, Amy L. Cole, Sixue Chen, and Alexander M. Cole (April 2011). reveals putative determinants of nasal carriage"Staphylococcus aureus"Exoproteome of . J Proteome Res 10 (4): 2064–78.  
  11. ^ http://www.gelifesciences.com/aptrix/upp00919.nsf/Content/17D93C2E6A580E57C1257628001CE677/$file/18114994AE.pdf
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