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Rhodamine B

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Title: Rhodamine B  
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Rhodamine B

Rhodamine B
IUPAC name
[9-(2-carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium chloride
Other names
Rhodamine 610, C.I. Pigment Violet 1, Basic Violet 10, C.I. 45170
ChemSpider  Y
Jmol-3D images Image
Molar mass 479.02
Appearance red to violet powder
Melting point 210 to 211 °C (410 to 412 °F; 483 to 484 K) (Decomposes)
~15 g/L (20 °C) [1]
Safety data sheet MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
 N  (: Y/N?)
An example of Beer–Lambert law. A green laser lighting a solution of rhodamine 6B; the beam becomes weaker as it travels through the dye.

Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.

Rhodamine B is used in biology as a Mycobacterium.

Rhodamine B is tunable around 610 nm when used as a laser dye.[2] Its luminescence quantum yield is 0.65 in basic ethanol,[3] 0.49 in ethanol,[4] 1.0,[5] and 0.68 in 94% ethanol.[6] The fluorescence yield is temperature dependent.[7]


  • Solubility 1
  • Synthesis 2
  • Other uses 3
  • Safety and health 4
  • References 5
  • See also 6


The solubility of Rhodamine B in water is ~15 g/L.[8] However, the solubility in acetic acid solution (30 vol.%) is ~400 g/L. Chlorinated tap water decomposes rhodamine B. Rhodamine B solutions are often absorbed in plastics therefore , it should be kept in glass.[9]


Rhodamine B synthesis

Other uses

Rhodamine B is being tested for use as a biomarker in oral rabies vaccines for wildlife, such as raccoons, to identify animals that have eaten a vaccine bait. The rhodamine is incorporated into the animal's whiskers and teeth.[10]

It is also often mixed with herbicides to show where they have been used.

Rhodamine B (BV10) is mixed with Quinacridone Magenta (PR122) to make the bright pink watercolor known as Opera Rose.[11]

Rhodamine is often used in colors illegally in order to obtain a graceful shade(Opera Rose) .

Safety and health

In California, rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label.[12]

In New Jersey, MSDS files state that there is limited evidence of carcinogenicity in laboratory animal, and no evidence at all in humans.[13]


  1. ^ Sicherheitsdatenblatt Rhodamin B by Roth, 2013
  2. ^ Rhodamine B
  3. ^ Kubin, R (1983). "Fluorescence quantum yields of some rhodamine dyes".  
  4. ^ Casey, Kelly G.; Quitevis, Edward L. (1988). "Effect of solvent polarity on nonradiative processes in xanthene dyes: Rhodamine B in normal alcohols". The Journal of Physical Chemistry 92 (23): 6590–6594.  
  5. ^ Kellogg, R. E.; Bennett, R. G. (1964). "Radiationless Intermolecular Energy Transfer. III. Determination of Phosphorescence Efficiencies". The Journal of Chemical Physics 41 (10): 3042.  
  6. ^ Snare, M (1982). "The photophysics of rhodamine B". Journal of Photochemistry 18 (4): 335–346.  
  7. ^ Karstens, T.; Kobs, K. (1980). "Rhodamine B and rhodamine 101 as reference substances for fluorescence quantum yield measurements". The Journal of Physical Chemistry 84 (14): 1871–1872.  
  8. ^ Sicherheitsdatenblatt Rhodamin B by Roth, 2013
  9. ^ Detection and prevention of leaks from dams By Antonio Plata Bedmar and Luís Araguás Araguás, Taylor & Francis, 2002, ISBN 90-5809-355-7
  10. ^ Slate, Dennis; Algeo, Timothy P.; Nelson, Kathleen M.; Chipman, Richard B.; Donovan, Dennis; Blanton, Jesse D.; Niezgoda, Michael; Rupprecht, Charles E. (2009). Bethony, Jeffrey M., ed. "Oral Rabies Vaccination in North America: Opportunities, Complexities, and Challenges". PLoS Neglected Tropical Diseases 3 (12): e549.  
  11. ^
  12. ^ Naval Jelly msds with Rhodamine B
  13. ^ J. T. Baker Rhodamine B MSDS

See also

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