World Library  
Flag as Inappropriate
Email this Article

Sudan Black B

Article Id: WHEBN0003191987
Reproduction Date:

Title: Sudan Black B  
Author: World Heritage Encyclopedia
Language: English
Subject: Sudan stain, Oil Red O, Stains, Gömöri trichrome stain, Movat's stain
Collection:
Publisher: World Heritage Encyclopedia
Publication
Date:
 

Sudan Black B

Sudan Black B
Names
IUPAC name
(2,2-dimethyl-1,3-dihydroperimidin-6-yl)-(4-phenylazo-1-naphthyl)diazene
Other names
Sudan Black; Fat Black HB; Solvent Black 3; C.I. 26150
Identifiers
 N
ChEBI  Y
ChemSpider  Y
Jmol-3D images Image
MeSH
PubChem
Properties
C29H24N6
Molar mass 456.54 g/mol
Melting point 120 to 124 °C (248 to 255 °F; 393 to 397 K)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
 N  (: Y/N?)

Sudan Black B (C29H24N6) is a nonfluorescent, relatively thermostable lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and melting point 120–124 °C. It stains blue-black.

Sudan Black B is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV.

Sudan Black B can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids.

A use of Sudan Black B is in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.

In differentiating haematological disorders Sudan black will stain myeloblasts but not lymphoblasts.

Production and composition

Sudan Black is formed by coupling of diazotized 4-phenylazonaphthalenamine-1 with 2,3-dihydro-2,2-dimethyl-1H-perimidine.[1] Therefore the main product expected was 2,3-dihydro-2,2dimethyl-6-[(4-phenylazo-1-naphthalenyl)-azo]-1H-perimidine. However the dye resulting from the above reaction product actually contains many, up to 42 colored and colorless by products that can be fractionated. The two major products were blue in color confirmed by various chromato-graphic (TLC and column etc.) separation and spectroscopic (IR, NMR, Mass) identification were named SSB-I & SSB-II (Rf values of 0.49 and 0.19 in thin Layer Chromatography).[2] The above described product indeed turned out to be SSB-II which comprises up to 60% of the mixture, and the SSB-I was 2,3-dihydro-2,2dimethyl-4-[(4-phenylazo-1-naphthalenyl)-azo]-1H-perimidine.


References

  1. ^ "Histochemistry. 1977 Dec 7;54(3):237-50. Sudan Black B: chemical structure and histochemistry of the blue main components. Pfüller U, Franz H, Preiss A". Springerlink.com. Retrieved 2012-05-25. 
  2. ^ HISTOCHEMISTRY AND CELL BIOLOGY Volume 16, Number 1 (1968), 68-84, DOI: 10.1007/BF00306212 Thin layer chrornatography and histochemistry of Sudan Black B A. G. W. Lansink

External links

  • Stains File entry
This article was sourced from Creative Commons Attribution-ShareAlike License; additional terms may apply. World Heritage Encyclopedia content is assembled from numerous content providers, Open Access Publishing, and in compliance with The Fair Access to Science and Technology Research Act (FASTR), Wikimedia Foundation, Inc., Public Library of Science, The Encyclopedia of Life, Open Book Publishers (OBP), PubMed, U.S. National Library of Medicine, National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health (NIH), U.S. Department of Health & Human Services, and USA.gov, which sources content from all federal, state, local, tribal, and territorial government publication portals (.gov, .mil, .edu). Funding for USA.gov and content contributors is made possible from the U.S. Congress, E-Government Act of 2002.
 
Crowd sourced content that is contributed to World Heritage Encyclopedia is peer reviewed and edited by our editorial staff to ensure quality scholarly research articles.
 
By using this site, you agree to the Terms of Use and Privacy Policy. World Heritage Encyclopedia™ is a registered trademark of the World Public Library Association, a non-profit organization.
 


Copyright © World Library Foundation. All rights reserved. eBooks from Project Gutenberg are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.