Wright's stain is a
histologic stain that facilitates the differentiation of
blood cell types. It is classically a mixture of
eosin (red) and
methylene blue dyes. It is used primarily to stain
peripheral blood smears and
bone marrow aspirates which are examined under a
light microscope. In
cytogenetics, it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases.
It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902. Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when infections are suspected.
The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify staining). The May-Grünwald stain, which produces a more intense coloration, also takes a longer time to perform.
White blood cells stained with Wright's stain:
See also
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