World Library  
Flag as Inappropriate
Email this Article

3T3 cells

Article Id: WHEBN0004372882
Reproduction Date:

Title: 3T3 cells  
Author: World Heritage Encyclopedia
Language: English
Subject: Non-terminally differentiated (blast) cells, Cell lines, RET proto-oncogene, Bioimage informatics, Homeobox protein NANOG
Collection:
Publisher: World Heritage Encyclopedia
Publication
Date:
 

3T3 cells

NIH/3T3 Fibroblasts in cell culture

3T3 cells come from a fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue.

Contents

  • Nomenclature 1
  • Characteristics 2
    • Expression 2.1
    • Cytogenetics 2.2
  • Uses 3
  • References 4
  • Notes 5

Nomenclature

The '3T3' designation refers to the abbreviation of "3-day transfer, inoculum 3 x 105 cells." This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 x 105 cells per 20-cm² dish (the second "3") continuously. The spontaneously immortalized cells with stable growth rate were established after 20-30 generations in culture, and then named '3T3' cells. Specifically, "3T3-L1" is one of the current line.

Characteristics

Swiss 3T3 can be inhibited by temazepam and other benzodiazepines. These cells are also contact inhibited. The cells are sensitive to sarcoma virus and leukemia virus focus formation. 3T3 cells can be transformed with SV40 and some other polyomaviruses.[1]

Expression

Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway.[2]

Cytogenetics

3T3 mouse cells are hypertriploid. The modal chromosome number is 68, which occurs in 30% of cells. Higher ploidies occur at a much lower rate of 2.4%.[1]

Uses

3T3 cells are often used in the cultivation of keratinocytes, with the 3T3 cells secreting growth factors favourable to these kinds of cells.


References

  • Todaro GJ, Green H . Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. Fulltext.
  • Nikon MicroscopyU Digital Video Gallery: 3T3 Cell Motility - a set of films of 3T3 cells in culture

Notes

  1. ^ a b http://nih3t3.com/
  2. ^ http://nih3t3.com/
This article was sourced from Creative Commons Attribution-ShareAlike License; additional terms may apply. World Heritage Encyclopedia content is assembled from numerous content providers, Open Access Publishing, and in compliance with The Fair Access to Science and Technology Research Act (FASTR), Wikimedia Foundation, Inc., Public Library of Science, The Encyclopedia of Life, Open Book Publishers (OBP), PubMed, U.S. National Library of Medicine, National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health (NIH), U.S. Department of Health & Human Services, and USA.gov, which sources content from all federal, state, local, tribal, and territorial government publication portals (.gov, .mil, .edu). Funding for USA.gov and content contributors is made possible from the U.S. Congress, E-Government Act of 2002.
 
Crowd sourced content that is contributed to World Heritage Encyclopedia is peer reviewed and edited by our editorial staff to ensure quality scholarly research articles.
 
By using this site, you agree to the Terms of Use and Privacy Policy. World Heritage Encyclopedia™ is a registered trademark of the World Public Library Association, a non-profit organization.
 


Copyright © World Library Foundation. All rights reserved. eBooks from Project Gutenberg are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.